10-2. Clostridium cellulovorans cellulosome and analysis by Bacillus subtilis heterologous host system
نویسندگان
چکیده
Studies about Clostridia have focused especially on Pathogens or cellulases. In this chapter we focus on Clostridium cellulovorans, a non-pathogenic Clostridium. C. cellulovorans is a cellulose degrader and produces an enzymatic complex called “cellulosome” in which several cellulases are bound tightly to a scaffolding protein. In addition to cellulosomes, C. cellulovorans produces free cellulases and hemicellulases called non-cellulosomal cellulases. Both kinds of cellulases degrade plant cell wall materials efficiently in a synergistic manner. We have succeeded to construct mincellulosomes using the B. subtilis system. Mega-cloning methods, the knowledge from B. subtilis studies, and the genome sequence of C. cellulovorans that has been determined recently will allow us to create cellulosomes that can be regulated at the
منابع مشابه
Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis, and preparation of active recombinant cellulosomes.
In cellulosomes produced by Clostridium spp., the high-affinity interaction between the dockerin domain and the cohesin domain is responsible for the assembly of enzymatic subunits into the complex. Thus, heterologous expression of full-length enzymatic subunits containing the dockerin domains and of the scaffolding unit is essential for the in vitro assembly of a "designer" cellulosome, or a r...
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